产品简介:
oen-step快速制胶试剂盒(10%)为一步法制胶试剂盒包含了配制SDS-PAGE凝胶所需的所有试剂。
产品组份:
A液80ml、B液80ml、C液250ml、D液250ml、凝胶溶剂8ml。
使用说明:
以0.75/1.0/1.5mm厚度的胶为例
下层胶配方 |
|
上层胶配方 |
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凝 胶厚度 |
C液 |
D液 |
凝胶剂 |
|
凝 胶厚度 |
A液 |
B液 |
凝胶剂 |
0.75mm |
2ml |
2ml |
40ul |
|
0.75mm |
0.5ml |
0.5ml |
10ul |
1.0mm |
2.7ml |
2.7ml |
60ul |
|
1.0mm |
0.75ml |
0.75ml |
15ul |
1.5mm |
4ml |
4ml |
80ul |
|
1.5mm |
1ml |
1ml |
20ul |
推荐电泳条件为:150V,约60min(或200V,约45min) |